W-010.06.131 [2021-03] Polycyclische aromatische Kohlenwasserstoffe (PAK) in Trinkwasser, Auswahltest zur Unterscheidung zwischen belasteten und unbelasteten Wässern

Aufgabenstellung/Zweck

This method presents a preliminary test with which those samples can be eliminated that contain less than 50 ng/l PAH (Limit value “TrinkwV 2001 (DE)”/German Drinking Water Ordinance: 100 ng/l). In terms of higher concentrations the quantitative determination according to  W-010.06.120 - Quantitative Bestimmung der Polycyclischen aromatischen Kohlenwasserstoffe (PAK) has to be carried out.

Anwendungsbereich

Drinking water intended for use in beer brewing as well as in the production of other foods.

The determination is conducted via HPLC. The analytical interest in those polycyclic arenes can be attributed to the emission into the environment of those toxic, partially carcinogenic substances from various emission sources. Polycyclic aromatic hydrocarbons (PAH) exist in almost all waters both undissolved adsorbed at solids (sediments, particulate matter) and dissolved in water. The differentiation between undissolved and dissolved PAH, which can be desirable for the analysis of surface water or wastewater, is not carried out during the drinking water analysis. The Drinking Water Ordinance postulates the determination of 5 polycyclic aromatic hydrocarbons, namely
    - Benzo-(b)-fluoranthene        - Benzo-(ghi)-perylene
    - Benzo-(a)-pyrene                  - Benzo-(k)-fluoranthene
    - Indeno-(1,2,3-cd)-pyrene

Prinzip

By the extraction with cyclohexane and evaporation the PAH are being fortified. The separation is carried out by high performance thin layer chromatography, the evaluation by examining under UV-light and comparison of the Rf-values with the reference substances.

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