Membrane filtration is a simple way of concentrating any microorganisms present in a non-turbid, liquid water sample.
Quantitative enrichment of large, filterable volumes on wort agar plates.
Membrane filters with pore sizes of 0.65 µm retain yeasts from the liquid sample, which are then incubated aerobically on agar nutrient media. The filtrate is collected in a receiver vessel and then discarded. After incubation, the yeast is analysed under a microscope.
The method describes the conditions for inoculating a culture medium.
Laboratories in the beverage industry in general and the brewing industry in particular.
The removal and addition of material from and to cultures, e.g. when inoculating culture media. Strict care must be taken to ensure that no foreign microorganisms enter the culture vessels or the inoculation material being transferred.