Detection of harmful yeasts and bacteria by means of membrane filtration and subsequent incubation on OFS agar.
Liquid sugar, filterable concentrates and raw materials.
A defined sample quantity is diluted, membrane-filtered, incubated and analysed.
Detection of fermentable yeasts and bacteria using the pour-plate method after prior liquid enrichment.
All non-filterable concentrates, bases and syrups.
The sample, which is pre-enriched in the SSL broth, is suspended in the culture medium (OFS agar), incubated and analysed.
Detection of fermentable yeasts and bacteria using a pour plate after prior liquid enrichment.
All cloudy beer-based beverages and lemonades.
The sample, which has been pre-enriched in SSL broth, is suspended in culture medium (OFS agar), incubated and analysed.
Mineral, spring and table waters as well as drinking waters that are to be marketed.
Qualitative detection of sulphite-reducing, spore-forming anaerobes in mineral water, spring water, table water, and other drinking water bottled for distribution to the consumer, by means of membrane filtration and placing the filter on a solid culture medium.
Note 1: If only the spores of sulphite-reducing anaerobes are to be analysed, the prescribed sample volume must be heated at 75 °C ± 1 °C for 10 minutes in order to inactivate the vegetative cells. Then cool quickly with cold water.
Important: Carry out a comparative test with the same volume of tap water to determine the total heating time required in the water bath.
Membrane filtration (MF) and placing the filter on dextrose-iron sulphate-sodium sulphite agar (DRCM agar) (corresponds to method Annex 2, point 4 a of § 4 para. 3 of the German Mineral and Table Water Ordinance, MTV) [1].
Mineral, spring and table waters as well as drinking waters that are to be marketed.
Qualitative detection of Escherichia coli and coliform bacteria in mineral, spring and table water, and other drinking water bottled for distribution to the consumer, by means of membrane filtration (MF) and placing the filter on endo agar [1].
This method describes how to determine whether a lot of barley or wheat is contaminated with Fusarium graminearum using mannitol agar.
Fusarium graminearum contamination in barley or wheat can be visually determined with the naked eye after three to five days of incubation on mannitol agar.