M-000.20.514 [2023-07] Brewery water – Membrane filtration for the analysis of yeasts using wort agar

Application/Purpose

Membrane filtration is a simple way of concentrating any microorganisms present in a non-turbid, liquid water sample.

Scope of Application

Quantitative enrichment of large, filterable volumes on wort agar plates.

Principle

Membrane filters with pore sizes of 0.65 µm retain yeasts from the liquid sample, which are then incubated aerobically on agar nutrient media. The filtrate is collected in a receiver vessel and then discarded. After incubation, the yeast is analysed under a microscope.

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