Detection of harmful yeasts and bacteria by means of membrane filtration and subsequent incubation on OFS agar.
All clear beer-based beverages and lemonades.
The sample is membrane-filtered, incubated and analysed.
Detection of fermentable yeasts and bacteria using a pour plate after prior liquid enrichment.
All cloudy beer-based beverages and lemonades.
The sample, which has been pre-enriched in SSL broth, is suspended in culture medium (OFS agar), incubated and analysed.
The method describes alternative culture media to OFS agar and SSL broth.
All beer-based beverages, lemonades, base products and sugar.
In addition to OFS agar and SSL broth, other culture media can be used as an alternative.
Determination of the concentration of dissolved carbon dioxide in carbonated beverages in bottles and cans
The total gas pressure in beer is measured after the beer has been forcefully shaken. The carbon dioxide is then bound through the addition of potassium hydroxide. The amount of air in the beer contributes the remaining volume of gas. Once the value for the total pressure has been corrected by subtracting the quantity of air present in the beer, the carbon dioxide can be measured [1].
Determination of the total air in bottles and cans
Determination of the total air in containers for beer, beer-based beverages and carbonated beverages
Through heating and shaking, the gases contained in beer are collected in a burette filled with potassium hydroxide. The carbon dioxide is bound by potassium hydroxide, and the remaining volume of gas, consisting of oxygen and nitrogen, is measured [1].
Determination of caffeine and theobromine using HPLC
This method is suitable for beverages containing caffeine, tea-based beverages and NAB.
Caffeine and theobromine are separated using HPLC and reversed phases and determined by means of UV detection.