This method describes how to determine whether a lot of barley or wheat is contaminated with Fusarium culmorum using mannitol agar to which malachite green has been added.
Barley intended for the production of malt; therefore, the kernels are to be evaluated on the basis of the characteristics described below. This method can also be utilized to evaluate wheat.
Fusarium culmorum contamination in barley or wheat can be visually determined with the naked eye after three to five days of incubation on mannitol agar to which malachite green has been added.