The method describes the conditions for DNA extraction.
Laboratories in the beverage industry in general and the brewing industry in particular.
DNA extraction for bacteria is performed according to a standard protocol as developed by Marmur [1] using lysozyme. A commercially available chitinase/zymolyase is used instead of lysozyme for filamentous fungi/yeasts. As an alternative to enzymatic lysis, the cells can be digested using several "freeze and thaw" cycles. Some fungi can only be broken down sufficiently by liquid nitrogen treatment. This involves freezing the cell material directly in liquid nitrogen and then grinding it with a mortar. The culture is frozen three times in liquid nitrogen and then heated and incubated for several hours in a lysis buffer.
Commercially available kits for DNA extraction can also be used as an alternative.