M-533.00 [2024-03] Analysis of non-alcoholic, cloudy, non-filterable beverages with a pH value < 4.3

Application/Purpose

Detection of harmful yeasts and bacteria in cloudy non-alcoholic beverages.

Scope of Application

All cloudy, non-filterable, non-alcoholic drinks with a pH value < 4.3.

Principle

For cloudy, non-filterable, non-alcoholic beverages such as juices, nectars, fruit juice drinks and lemonades, the relevant beverage spoilers (in particular fermentable yeasts, respiratory yeasts, lactic and acetic acid bacteria) should be detected in accordance with the methods:

M-533.60.502 Cloudy, non-filterable, non-alcoholic beverages with a pH value < 4.3 – Detection of beverage spoilers using the pour-plate method

M-533.60.517 Cloudy, non-filterable, non-alcoholic beverages with pH < 4.3 – Two-stage enrichment of beverage spoilers

Anaerobic incubation is recommended for carbonated beverages in order to increase the selectivity of the analysis for this type of beverage.

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