M-020.09.506 [2023-07] Mineral, spring and table water, testing for E. coli and coliform bacteria using double-concentrated DEV lactose peptone broth

Application/Purpose

The method describes the determination of E. coli and coliform bacteria in mineral, spring and table water using double-concentrated DEV lactose peptone broth (based on Annex 2, Section 1.1a/1.2a to Section 4 (3) of the German Mineral and Table Water Ordinance of 1 August 1984, as amended on 5 July 2017).

Scope of Application

Mineral, spring and table waters, as well as drinking waters that are to be marketed.

Principle

Qualitative detection of Escherichia coli and coliform bacteria using enrichment culture with double-concentrated lactose broth [1]. E. coli and coliform bacteria break down lactose, producing gas and acid.

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