B-590.25.111 [2020-10] Cyclamate – Photometric Method

The following artificial sweeteners are used in reduced-calorie, non-alcoholic beverages made with a base of fruit juice and can be found in the guidelines for sweeteners (VO 1333/2008 EG):

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Application/Purpose

Determination of the sweetener cyclamate using photometry

Scope of Application

Suitable for beer-based beverages and NAB

Principle

Cyclamate reacts with sodium hypochlorite to form N,N-dichlorocyclohexylamine. The chlorine compounds are isolated through extraction with heptane and are washed free using excess chlorine. The UV absorption is measured at 314 nm.

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