B-590.13.134 [2020-10] Fermentable Carbohydrates – HPLC

Application/Purpose

Analysis of the sugar spectrum in all beverages

Principle

Fructose, glucose as well as disaccharides and trisaccharides (maltose and maltotriose, DP 2 and DP 3) are determined using high-pressure liquid chromatography (HPLC).

Wort or beer is deionized using an ion exchanger. The sample is filtered and concentrated using a solid-phase column and analyzed chromatographically. The concentration of the sugars present in the sample is calculated from the chromatograms obtained by analysis of the calibration solutions.

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