This method describes how to determine whether a lot of barley or wheat is contaminated with Fusarium graminearum using mannitol agar.
Barley intended for the production of malt; therefore, the kernels are to be evaluated on the basis of the characteristics described below. This method can also be utilized to evaluate wheat.
Fusarium graminearum contamination in barley or wheat can be visually determined with the naked eye after three to five days of incubation on mannitol agar.