B-420.20.131 [2020-10] 4-Vinylguaiacol and 4-Vinylphenol – HPLC

Aromatic alcohols are compounds which exhibit a strong sensory influence on beer. This is especially true of some top-fermented beers with the pronounced aroma of guaiacol. Phenolcarboxylic acids are precursors of these substances and, in fact, possess antioxidative properties.

4-Vinylguaiacol and 4-vinylphenol are derivatives of ferulic acid and coumaric acid, respectively. Both substances appear in higher concentrations in Southern German wheat beers (Weissbier). 4-Vinyl guaiacol is considered an indicator substance.

Application/Purpose

Determination of 4-vinylguaiacol and 4-vinylphenol in beer

Scope of Application

The method is suitable for beer brewed to all ranges of original gravity and alcohol content.

Principle

Analysis was performed using isocratic HPLC on a Spherisorb separation column with UV detection at 260 nm.

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